The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells

• Published in: International journal of molecular sciences 2019-12, Vol.20 (23), p.6099
• Main Authors: Mucha, Joanna, Pawłowski, Tomasz A, Klupczyńska, Ewelina A, Guzicka, Marzenna, Zadworny, Marcin
• Format: Article
• Language: English
• Subjects: Acetylation; Alternations; Apoptosis; Cell Death; Cell division; Chelation; Confocal microscopy; Cytology; Cytoskeleton; Depolymerization; Enzymes; Fungi; Gene expression; Histone H3; Histones; Histones - metabolism; Hydroxamic Acids - chemistry; Iron; Metabolism; Metabolites; Metabolome; Microtubules - metabolism; Mycorrhizae - metabolism; Mycorrhizae - pathogenicity; Pathogens; Pinus sylvestris; Pinus sylvestris - cytology; Plant resistance; Plant roots; Plant Roots - cytology; Plant Roots - metabolism; Plant Roots - microbiology; Proteins; Siderophores; Siderophores - chemistry; Tubulin; Tubulin - metabolism; Western blotting
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20236099

Protein acetylation affects gene expression, as well as other processes in cells, and it might be dependent on the availability of the metals. However, whether iron chelating compounds (siderophores) can have an effect on the acetylation process in plant roots is largely unknown. In the present study, western blotting and confocal microscopy was used to examine the degree of acetylation of histone H3 and alpha tubulin in root cells in the presence of structurally different siderophores. The effect of metabolites that were produced by pathogenic and mycorrhizal fungi was also assessed. No effect was observed on histone acetylation. By contrast, the metabolites of the pathogenic fungus were able to decrease the level of microtubule acetylation, whereas treatment with iron-free ferrioxamine (DFO) was able to increase it. This latter was not observed when ferrioxamine-iron complexes were used. The pathogen metabolites induced important modifications of cytoskeleton organization. Siderophores also induced changes in the tubulin skeleton and these changes were iron-dependent. The effect of siderophores on the microtubule network was dependent on the presence of iron. More root cells with a depolymerized cytoskeleton were observed when the roots were exposed to iron-free siderophores and the metabolites of pathogenic fungi; whereas, the metabolites from mycorrhizal fungi and iron-enriched forms of siderophores slightly altered the cytoskeleton network of root cells. Collectively, these data indicated that the metabolites of pathogenic fungi mirror siderophore action, and iron limitation can lead to enhanced alternations in cell structure and physiology.