Kinetic of Adhesion of S. epidermidis with Different EPS Production on Ti6Al4V Surfaces

Controlling initial bacterial adhesion is essential to prevent biofilm formation and implant-related infection. The search for surface coatings that prevent initial adhesion is a powerful strategy to obtain implants that are more resistant to infection. Tracking the progression of adhesion on surfac...

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Bibliographic Details
Published in:BioMed research international 2019, Vol.2019 (2019), p.1-8
Main Authors: Gallardo-Moreno, Amparo M., González-Martín, M. Luisa, Rodríguez-Cano, Abraham, Pacha-Olivenza, Miguel Ángel
Format: Article
Language:English
Subjects:
Adhesion
Alloys
Analysis
Antibiotics
Bacteria
Bacterial Adhesion - physiology
Biofilms
Coatings
Development and progression
Extracellular Polymeric Substance Matrix - metabolism
Flow chambers
Health aspects
Implants
Infection
Infections
Kinetics
Laminar flow
Metabolism
Parallel plate flow cells
Parameters
Production capacity
Staphylococcus epidermidis
Staphylococcus epidermidis - metabolism
Staphylococcus epidermidis - physiology
Substrates
Surface properties
Titanium - chemistry
Titanium alloys
Titanium base alloys
Transplants & implants
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Summary:Controlling initial bacterial adhesion is essential to prevent biofilm formation and implant-related infection. The search for surface coatings that prevent initial adhesion is a powerful strategy to obtain implants that are more resistant to infection. Tracking the progression of adhesion on surfaces from the beginning of the interaction between bacteria and the surface provides a deeper understanding of the initial adhesion behavior. To this purpose, we have studied the progression over time of bacterial adhesion from a laminar flow of a bacterial suspension, using a modified Robbins device (MRD). Comparing with other laminar flow devices, such as the parallel plate flow chamber, MRD allows the use of diverse substrata under the same controlled flow conditions simultaneously. Two different surfaces of Ti6Al4V and two strains of Staphylococcus epidermidis with different exopolymer production were tested. In addition, the modified Robbins device was examined for its convenience and suitability for the purpose of this study. Results were analyzed according to a pseudofirst order kinetic. The values of the parameters obtained from this model make it possible to discriminate the adhesive behavior of surfaces and bacteria. One of the fitting parameters depends on the bacterial strain and the other only on the surface properties of the substrate.
ISSN:2314-6133
2314-6141
2314-6141
DOI:10.1155/2019/1437806