Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils

To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore p...

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Bibliographic Details
Published in:Microbes and Environments 2019, Vol.34(4), pp.347-355
Main Authors: Habibi, Safiullah, Djedidi, Salem, Ohkama-Ohtsu, Naoko, Sarhadi, Wakil Ahmad, Kojima, Katsuhiro, Rallos, Roland V., Ramirez, Maria Daniela Artigas, Yamaya, Hiroko, Sekimoto, Hitoshi, Yokoyama, Tadashi
Format: Article
Language:English
Subjects:
16S rRNA
Acetic acid
Acetylene
Acetylene reduction
Acid production
Alkaline soils
ARA
Bacteria
Biofertilizers
Cultivars
Fertilizers
Gene sequencing
Genetic diversity
Genetic variation
IAA
Indoleacetic acid
Inoculation
Nitrogen fixation
Nitrogenase
Nitrogenation
PGPR
phosphate and potassium solubilization
Phosphates
Physical characteristics
Plant growth
Potassium
Rice
rRNA 16S
Soil
Soils
Solubilization
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Summary:To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL−1) and exhibited high nitrogenase activity (647.4 nmol C2H4 h−1), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.ME18168