Chinese Herbal Medicine Formula Shenling Baizhu San Ameliorates High-Fat Diet-Induced NAFLD in Rats by Modulating Hepatic MicroRNA Expression Profiles

Objective. The purpose of present study was to investigate the potential mechanism underlying the protective effect of Shenling Baizhu San (SLBZS) on nonalcoholic fatty liver disease (NAFLD) by microRNA (miRNA) sequencing. Methods. Thirty male Wistar rats were randomly divided into a normal control...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2019, Vol.2019 (2019), p.1-14
Main Authors: Yang, Qin-He, Tang, Kairui, Nie, Huan, Zheng, Chuiyang, Deng, Yuanjun, Pan, Maoxing, Zhang, Yu-Pei
Format: Article
Language:English
Subjects:
Bioengineering
Bioinformatics
Cholesterol
Diet
Fatty acids
Fatty liver
Herbal medicine
High fat diet
Laboratory animals
Liver
Liver diseases
Medicine, Botanic
Medicine, Chinese
Medicine, Herbal
Metabolic pathways
Metabolism
MicroRNA
MicroRNAs
miRNA
Pathogenesis
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Summary:Objective. The purpose of present study was to investigate the potential mechanism underlying the protective effect of Shenling Baizhu San (SLBZS) on nonalcoholic fatty liver disease (NAFLD) by microRNA (miRNA) sequencing. Methods. Thirty male Wistar rats were randomly divided into a normal control (NC) group, a high-fat diet (HFD) group, and an SLBZS group. After 12 weeks, the biochemical parameters and liver histologies of the rats were assessed. The Illumina HiSeq 2500 sequencing platform was used to analyse the hepatic miRNA expression profiles. Representative differentially expressed miRNAs were further validated by qRT-PCR. The functions of the differentially expressed miRNAs were analysed by bioinformatics. Results. Our results identified 102 miRNAs that were differentially expressed in the HFD group compared with the NC group. Among those differentially expressed miRNAs, the expression levels of 28 miRNAs were reversed by SLBZS administration, suggesting the modulation effect of SLBZS on hepatic miRNA expression profiles. The qRT-PCR results confirmed that the expression levels of miR-155-5p, miR-146b-5p, miR-132-3p, and miR-34a-5p were consistent with those detected by sequencing. Bioinformatics analyses indicated that the target genes of the differentially expressed miRNAs reversed by SLBZS were mainly related to metabolic pathways. Conclusion. This study provides novel insights into the mechanism of SLBZS in protecting against NAFLD; this mechanism may be partly related to the modulation of hepatic miRNA expression and their target pathways.
ISSN:1741-427X
1741-4288
DOI:10.1155/2019/8479680