Association of Common Variants in HNF1A Gene with Serum AFP Level in Healthy Chinese Individuals and HCC Patients

• Published in: Disease markers 2019, Vol.2019 (2019), p.1-11
• Main Authors: Liang, Guo-wei, He, Mei-lin, Shao, Dong-hua, Li, Xue-jun
• Format: Article
• Language: English
• Subjects: Adult; AFP gene; Alcohol; alpha-Fetoproteins - metabolism; Antigens; Binding sites; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Blood pressure; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cardiovascular disease; Case-Control Studies; Cholesterol; Coronary vessels; Deoxyribonucleic acid; Diabetes; Diabetic retinopathy; Diagnosis, Differential; Differential diagnosis; DNA; Fasting; Female; Gene polymorphism; Genetic Association Studies; Genetic diversity; Genetic Predisposition to Disease; Genetic variance; Genomes; Genotype; Genotypes; Hepatitis; Hepatocellular carcinoma; Hepatocyte Nuclear Factor 1-alpha - genetics; HNF1a gene; Hospitals; Humans; Levels; Liver cancer; Liver diseases; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Male; Medical diagnosis; Metastasis; Middle Aged; Molecular biology; Mutation; Nucleotides; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Single-nucleotide polymorphism; Transcription factors; Tumor markers; Tumors; α-Fetoprotein
• ISSN: 0278-0240; 1875-8630
• DOI: 10.1155/2019/6273497

Although alpha-fetoprotein (AFP) is a widely used tumor marker in hepatocellular carcinoma (HCC), 40% of newly diagnosed patients do not have an elevated AFP level. Research has revealed that mutations in the HNF1A binding site of the AFP gene promoter cause significantly elevated serum AFP levels in patients with hereditary persistence of AFP. This study investigated the relationship between HNF1A genetic variants and serum AFP levels. We examined the association between the HNF1A-rs1169288 (A/C), rs2464196 (G/A), and rs1169310 (C/T) polymorphisms and AFP levels in a healthy Chinese population (n=1010) and HCC patients (n=185). Single nucleotide polymorphisms were genotyped by the amplification refractory mutation system combined with TaqMan probe in real-time PCR. The serum AFP concentrations were measured using the Architect i2000 immunochemistry analyzer. In healthy individuals, serum AFP levels were significantly lower with the rs2464196-AA and rs1169310-TT genotypes. Similar significant differences were observed in HCC patients. Moreover, in HCC patients, the distribution frequencies of rs2464196-AA+AG and rs1169310-TT+TC among those with AFP≤20 ng/ml or ≤400 ng/ml were significantly lower than those in patients with AFP>20 ng/ml or >400 ng/ml. Among all subjects, those carrying the HNF1A-rs2464196-A or rs1169310-T allele tended to have low levels of AFP. However, the HNF1A-rs1169288 polymorphism showed no significant association with the serum AFP level. These findings provide new insight into the genetic determinants of serum AFP level and can aid the differential diagnosis of HCC patients with low serum AFP.