Polarization Sensing of Fluorophores in Tissues for Drug Compliance Monitoring

We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a...

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Bibliographic Details
Published in:Analytical biochemistry 1999-09, Vol.273 (2), p.204-211
Main Authors: Gryczynski, Zygmunt, Abugo, Omoefe O., Lakowicz, Joseph R.
Format: Article
Language:English
Subjects:
Animals
Chickens
Drug Monitoring - instrumentation
Evaluation Studies as Topic
Fluorescence Polarization - instrumentation
Fluorescent Dyes - analysis
Humans
Lasers
Models, Theoretical
Patient Compliance
Rhodamines - analysis
Tissue Distribution
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Summary:We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a highly polarized reference film and the unpolarized or orthogonally polarized emission of Rh800 from the scattering intralipid or tissue. The concentration of Rh800 in this medium was revealed by large changes in the polarization (P) with values of P ranging from 0.8 to −0.9. It is possible to vary the sensitive Rh800 concentration range by variation of the detected emission wavelengths, orientation of the excitation polarizer, or fluorophore concentration in the reference film. Polarization sensing of fluorophores in tissue requires only steady-state detection, and can be accomplished with simple and/or portable electronics. Such devices may find use in electronic detection of ingested medicines based on transdermal detection of nontoxic long-wavelength fluorophores.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1999.4213