miR-520b Promotes Breast Cancer Stemness Through Hippo/YAP Signaling Pathway

The breast cancer stem cells contribute to the initiation, progression, recurrence, metastasis as well as resistance of breast cancer. However, the mechanisms underlying the maintenance of breast cancer stemness have not been fully understood. TCGA and GEO data were used for measuring miR-520b expre...

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Bibliographic Details
Published in:OncoTargets and therapy 2019-12, Vol.12, p.11691-11700
Main Authors: Zhang, Hui, Lang, Ting-Yuan, Zou, Dong-Ling, Zhou, Lei, Lou, Meng, Liu, Jing-Shu, Li, Yun-Zhe, Ding, Dong-Yan, Li, Yu-Cong, Zhang, Na, Zheng, Xiao-Dong, Zeng, Xiao-Hua, Zhou, Qi, Li, Li
Format: Article
Language:English
Subjects:
Analysis
Anopheles
Breast cancer
Cancer cells
Cancer metastasis
Development and progression
Genes
Genetic engineering
Medical research
Messenger RNA
Original Research
Prognosis
RNA
Scientific equipment industry
Stem cells
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Summary:The breast cancer stem cells contribute to the initiation, progression, recurrence, metastasis as well as resistance of breast cancer. However, the mechanisms underlying the maintenance of breast cancer stemness have not been fully understood. TCGA and GEO data were used for measuring miR-520b expression in breast cancer tissues. Kaplan-meier analysis was used for determining the relationship between miR-520b expression level and the prognosis of patients. Genetic manipulation was performed by lentivirus system and miR-520b inhibitor was used for knockdown of miR-520b. qRT-PCR and Western blot were employed to determine the mRNA and protein levels, respectively. The stemness and EMT (Epithelial to mesenchymal transition) were assessed by sphere-formation and transwell assay as well as the expression of the related markers. The target genes of miR-520b were identified using the online database starBase V3.0. miR-520b is upregulated in cancer tissues of breast cancer patients and predicts poor prognosis. Upregulation of miR-520b was found in breast cancer stem cells. Ectopic expression of miR-520b promotes the stemness of the breast cancer cells, conversely, depletion of miR-520b attenuates the stemness of these cells. miR-520b positively regulates Hippo/YAP signaling pathway and overexpression of LAST2 abolished the effect of miR-520b on the stemness of breast cancer cells. miR-520b promotes the stemness of breast cancer patients by activating Hippo/YAP signaling via targeting LATS2.
ISSN:1178-6930
1178-6930
DOI:10.2147/ott.s236607