Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood

We have determined the fluorescence characteristics of two long wavelength dyes, albumin blue 633 (AB633) and 670 (AB670), in plasma and blood to evaluate the possibility of making direct fluorescence sensing measurements in blood. Using binding and lifetime measurements we were also able to show th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Biomedical Optics 2001-07, Vol.6 (3), p.359-365
Main Authors: Abugo, Omoefe O, Herman, Petr, Lakowicz, Joseph R
Format: Article
Language:English
Subjects:
Anisotropy
Benzoxazoles - blood
Blood
Blood Proteins - metabolism
Cells
Cyclopentanes - blood
Dyes
Fluorescence
Fluorescent Dyes - metabolism
Hemoglobin
Humans
Nitriles - blood
plasma
Plasma - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have determined the fluorescence characteristics of two long wavelength dyes, albumin blue 633 (AB633) and 670 (AB670), in plasma and blood to evaluate the possibility of making direct fluorescence sensing measurements in blood. Using binding and lifetime measurements we were also able to show that these dyes bind selectively to human serum albumin (HSA) in plasma and blood. By measuring changes in the mean lifetime of AB670 with changes in the HSA concentration, we showed that lifetime-based sensing can be used to monitor HSA concentrations using these albumin blue dyes. Anisotropy measurements for AB633 and AB670 in plasma and blood revealed high anisotropy values for these dyes in these media. Exploiting these high anisotropies, we were also able to determine HSA concentrations in plasma and blood mimics using changes in AB670 anisotropy with HSA concentration. These results show that, apart from being able to make fluorescence measurements directly in plasma and blood, it is possible to sense directly for specific plasma/blood components using fluorescent probes that bind preferentially to them. ©
ISSN:1083-3668
1560-2281
DOI:10.1117/1.1381053