Extraction and Determination of Polar Bioactive Compounds from Alfalfa ( Medicago sativa L.) Using Supercritical Techniques

The aim of this research was to select parameters for supercritical extraction with CO of L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the p...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-12, Vol.24 (24), p.4608
Main Authors: Wrona, Olga, Rafińska, Katarzyna, Walczak-Skierska, Justyna, Możeński, Cezary, Buszewski, Bogusław
Format: Article
Language:English
Subjects:
Alfalfa
Bioactive compounds
Biological Products - chemistry
Biological Products - isolation & purification
Biological Products - pharmacology
Carbon dioxide
Chemical Fractionation
Chromatography
Chromatography, Supercritical Fluid
Daidzein
Ethanol
Flavonoids
Food
Genistein
Investigations
Medicago sativa - chemistry
Phenols
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Quadrupoles
Solvents
Vapor pressure
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Summary:The aim of this research was to select parameters for supercritical extraction with CO of L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the purpose of optimization, mathematical statistics was used. Qualitative analysis of products was performed with supercritical fluid chromatography (SFC). The SFC analysis revealed a proper separation of flavonoids and phenolics acids for dedicated TFC and TPC optimal parameters. The obtained results have proved that it is a possibility to extract polar compounds with non-polar solvent under higher values of pressure and temperature and to enrich product with desired group of bioactive compounds with proper optimization. The proposed extraction technique allows to obtain on an industrial scale, using an environmentally friendly solvent, a preparation rich in biologically active nutrients that can be implemented in the cosmetics, pharmaceutical and food industries.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24244608