Changes to the TDP-43 and FUS Interactomes Induced by DNA Damage

• Published in: Journal of proteome research 2020-01, Vol.19 (1), p.360-370
• Main Authors: Kawaguchi, Tetsuya, Rollins, Matthew G, Moinpour, Mahta, Morera, Andres A, Ebmeier, Christopher C, Old, William M, Schwartz, Jacob C
• Format: Article
• Language: English
• Subjects: DNA Damage; DNA Repair; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; HEK293 Cells; Humans; Immunoprecipitation; Protein Interaction Maps; RNA-Binding Protein FUS - genetics; RNA-Binding Protein FUS - metabolism
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/acs.jproteome.9b00575

The RNA-binding proteins TDP-43 and FUS are tied as the third leading known genetic cause for amyotrophic lateral sclerosis (ALS), and TDP-43 proteopathies are found in nearly all ALS patients. Both the natural function and contribution to pathology for TDP-43 remain unclear. The intersection of functions between TDP-43 and FUS can focus attention for those natural functions mostly likely to be relevant to disease. Here, we compare the role played by TDP-43 and FUS, maintaining chromatin stability for dividing HEK293T cells. We also determine and compare the interactomes of TDP-43 and FUS, quantitating changes in those before and after DNA damage. Finally, selected interactions with known importance to DNA damage repair were validated by co-immunoprecipitation assays. This study uncovered TDP-43 and FUS binding to several factors important to DNA repair mechanisms that can be replication-dependent, -independent, or both. These results provide further evidence that TDP-43 has an important role in DNA stability and provide new ways that TDP-43 can bind to the machinery that guards DNA integrity in cells.