Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

• Published in: Evidence-based complementary and alternative medicine 2019, Vol.2019 (2019), p.1-20
• Main Authors: Qian, Yu-liang, Wang, Ning, Zhang, Zi-han, Qian, Hui, Yue, Bo-wen, Zhao, Li-li, Yan, Dong, Yan, Shi-Hai
• Format: Article
• Language: English
• Subjects: Angina; Angina pectoris; Angiotensin; Angiotensin II; Blood; Cardiac patients; Cardiomyocytes; Cardiovascular disease; Cardiovascular diseases; Cell injury; Cell lines; Chinese medicine; Coronary artery disease; Coronary vessels; Cyclic AMP; Cytoplasm; Energy metabolism; Enzyme-linked immunosorbent assay; Erythrocytes; Fibrinogen; GABARAP protein; Granular materials; Heart; Heart beat; Heart diseases; Heart rate; High density lipoprotein; Inflammation; Interleukin 6; Internal medicine; Ischemia; Low density lipoprotein; Low density lipoproteins; Medical treatment; Molecular modelling; Myocardial ischemia; Na+/K+-exchanging ATPase; Oxidation; Oxidative stress; Patients; Physiology; Rigidity; Smooth muscle; Staining; Viscosity
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2019/1808419

Objective. Coronary heart disease (CHD) has been regarded as a serious and common disease in the modern society. This study aims to investigate the effect of Granule of BU-XIN RUAN-MAI (BXRM) on angina pectoris of coronary heart disease and to explore the molecular mechanisms underlying Granule of BU-XIN RUAN-MAI-mediated protective activity against this disease. Methods. The effects of Granule of BU-XIN RUAN-MAI on clinical symptoms of patients’ angina were indicated by hemorheology indicators including high shear of blood viscosity, low shear of blood viscosity, plasma viscosity, erythrocyte rigidity index, D-D dimer, fibrinogen content, and lipid content. The effects of Granule of BU-XIN RUAN-MAI on isoprenaline-induced myocardial cell injury were determined by conducting H&E staining and by performing ELISA to examine the serum content of MDA, SOD, Na+/K+-ATPase, cAMP, and the content of inflammatory factors in isoprenaline-induced rats. Meanwhile, western blot and real time PCR were used to determine the expression of genes involved in oxidation and energy metabolism, and real time PCR was also used for determination of miR-542-3p expression. Luciferase reporter assay was conducted to test the binding sites of miR-542-3p on GABARAP 3′UTR. The chemical compositions of Granule of BU-XIN RUAN-MAI were determined by liquid LC-QTOF-MS. Results. Granule of BU-XIN RUAN-MAI significantly attenuated the clinical symptoms of patients’ angina by improving the patients’ heart rate and by decreasing the level of hemorheology indicators and also by reducing the serum content of TC, TG, LDL, and elevated HDL content. H&E staining demonstrated that Granule of BU-XIN RUAN-MAI ameliorated the myocardial ischemia in a dose-dependent manner. Besides, Granule of BU-XIN RUAN-MAI downregulated serum MDA content and upregulated the content of SOD, Na+/K+-ATPase, and cAMP in isoprenaline-induced rats. Granule of BU-XIN RUAN-MAI significantly improved oxidation stress by increasing PPARα expression, and it inhibited inflammation by downregulating expression and contents of IL-6, IL-1β, and TNF-α. Then, Granule of BU-XIN RUAN-MAI-containing serum increased the SOD content, and reduced the MDA content in angiotensin II-stimulated HUVEC cells. The granule of BU-XIN RUAN-MAI-containing serum obviously downregulated protein expressions of P40phox, P47phox, and P67phox in plasma membrane, and it significantly increased protein levels of P40phox, P47phox, and P67phox in the cytoplasm