Selection and functional identification of a synthetic partial ABA agonist, S7

The stress hormone abscisic acid (ABA) helps plants to survive under abiotic stresses; however, its use as an agrochemical is limited by its chemical instability and expense. Here, we report the development of an in vivo screening system to isolate chemicals able to induce ABA signalling responses i...

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Bibliographic Details
Published in:Scientific reports 2020-01, Vol.10 (1), p.4, Article 4
Main Authors: Min, Myung Ki, Kim, Rigyeong, Moon, Seok-Jun, Lee, Yongsang, Han, Seungsu, Lee, Sangho, Kim, Beom-Gi
Format: Article
Language:English
Subjects:
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631/449/2675
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96/95
96/98
Abscisic acid
Abscisic Acid - pharmacology
Agonists
Agrochemicals
Aquatic plants
Gene expression
Gene Expression Regulation, Plant - drug effects
High-Throughput Screening Assays
Humanities and Social Sciences
Molecular weight
multidisciplinary
Oryza - drug effects
Oryza - physiology
Oryza sativa
Plant Growth Regulators - pharmacology
Plant Proteins - genetics
Plant Proteins - metabolism
Promoter Regions, Genetic
Protoplasts
Reporter gene
Science
Science (multidisciplinary)
Seed germination
Seedlings
Seedlings - drug effects
Seedlings - physiology
Small Molecule Libraries - pharmacology
Stress, Physiological
Water loss
Yeasts
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Summary:The stress hormone abscisic acid (ABA) helps plants to survive under abiotic stresses; however, its use as an agrochemical is limited by its chemical instability and expense. Here, we report the development of an in vivo screening system to isolate chemicals able to induce ABA signalling responses in rice ( Oryza sativa ) protoplasts. This system consists of an ABA-hypersensitive synthetic promoter containing ABRE and DRE motifs driving a luciferase reporter gene. After efficiently transfecting rice protoplasts with this construct, we screened chemicals library with a similar molecular weight and chemical structure to ABA and identified one chemical, S7, that induced ABA signalling by mediating interactions between the group I and II OsPYL receptors and certain OsPP2CAs in a yeast two-hybrid assay. In an in vitro pulldown assay, S7 was found to mediate a weak interaction between OsPYL5/8 and various OsPP2CAs. S7 treatments did not affect seedling growth or seed germination, but could reduce water loss. Rice seedlings treated with S7 exhibited transcriptome profiles that partially overlapped those treated with ABA. Taken together, we concluded that S7 is a new partial ABA agonist, which has potential use in future dissections of ABA signalling and as an agrochemical.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-56343-9