RNU6B, a frequent reference in miRNA expression studies, differentiates between deaths caused by hypothermia and chronic cardiac ischemia

Here, we tested the usefulness of small non-coding RNAs as references in quantitative RT-PCR expression analyses in hypothermia and chronic cardiac ischemia as the primary causes of death. Cq values of RNU6B, SCARNA17, SNORD25, and SNORA73A were determined from human cadaver samples of hypothermia a...

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Bibliographic Details
Published in:International journal of legal medicine 2020-01, Vol.134 (1), p.159-162
Main Authors: Kaija, Helena, Pakanen, Lasse, Porvari, Katja
Format: Article
Language:English
Subjects:
Autopsies
Biomarkers - analysis
Cadaver
Cadavers
Cardiovascular disease
Cause of Death
Fatalities
Forensic Medicine
Forensic pathology
Gene expression
Heart
Heart diseases
Hospitals
Humans
Hypothermia
Hypothermia - genetics
Ischemia
Medical Law
Medical research
Medicine
Medicine & Public Health
MicroRNAs
Myocardial Ischemia - genetics
Physiology
Real-Time Polymerase Chain Reaction
Reference Values
RNA Stability
RNA, Small Untranslated - analysis
Short Communication
Trauma
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Summary:Here, we tested the usefulness of small non-coding RNAs as references in quantitative RT-PCR expression analyses in hypothermia and chronic cardiac ischemia as the primary causes of death. Cq values of RNU6B, SCARNA17, SNORD25, and SNORA73A were determined from human cadaver samples of hypothermia and cardiac deaths. Average Cq values of RNU6B were higher in hypothermic and average SCARNA17 Cq values in chronic ischemic samples, but no difference in SNORD25 and SNORA73A Cq values could be seen between the groups. RNU6B expression levels were calculated using SNORD25, SNORA73A, and their combination as the reference in normalization. Expression of RNU6B, a widely used reference, was found to be significantly lower in hypothermia than in chronic cardiac ischemia. In these conditions, RNU6B is a useful marker differentiating hypothermia deaths from chronic ischemic heart disease deaths, but not a valid reference for normalization in expression studies.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-019-02041-0