Detection of Multiple Variants of Grapevine Fanleaf Virus in Single Xiphinema index Nematodes

Grapevine fanleaf virus (GFLV) is responsible for a widespread disease in vineyards worldwide. Its genome is composed of two single-stranded positive-sense RNAs, which both show a high genetic diversity. The virus is transmitted from grapevine to grapevine by the ectoparasitic nematode . Grapevines...

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Bibliographic Details
Published in:Viruses 2019-12, Vol.11 (12), p.1139
Main Authors: Garcia, Shahinez, Hily, Jean-Michel, Komar, Véronique, Gertz, Claude, Demangeat, Gérard, Lemaire, Olivier, Vigne, Emmanuelle
Format: Article
Language:English
Subjects:
Bioinformatics
Disease transmission
Ectoparasites
Genetic diversity
Genomes
Life Sciences
Microbiology and Parasitology
Mutation
Nematodes
Phylogenetics
Phylogeny
Phytopathology and phytopharmacy
Ribonucleic acid
RNA
RNA polymerase
Vegetal Biology
Vineyards
Virology
Viruses
Wineries & vineyards
Xiphinema index
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Summary:Grapevine fanleaf virus (GFLV) is responsible for a widespread disease in vineyards worldwide. Its genome is composed of two single-stranded positive-sense RNAs, which both show a high genetic diversity. The virus is transmitted from grapevine to grapevine by the ectoparasitic nematode . Grapevines in diseased vineyards are often infected by multiple genetic variants of GFLV but no information is available on the molecular composition of virus variants retained in following nematodes feeding on roots. In this work, aviruliferous were fed on three naturally GFLV-infected grapevines for which the virome was characterized by RNAseq. Six RNA-1 and four RNA-2 molecules were assembled segregating into four and three distinct phylogenetic clades of RNA-1 and RNA-2, respectively. After 19 months of rearing, single and pools of 30 tested positive for GFLV. Additionally, either pooled or single carried multiple variants of the two GFLV genomic RNAs. However, the full viral genetic diversity found in the leaves of infected grapevines was not detected in viruliferous nematodes, indicating a genetic bottleneck. Our results provide new insights into the complexity of GFLV populations and the putative role of as reservoirs of virus diversity.
ISSN:1999-4915
1999-4915
DOI:10.3390/v11121139