Plant gene editing through de novo induction of meristems

Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, tim...

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Bibliographic Details
Published in:Nature biotechnology 2020-01, Vol.38 (1), p.84-89
Main Authors: Maher, Michael F., Nasti, Ryan A., Vollbrecht, Macy, Starker, Colby G., Clark, Matthew D., Voytas, Daniel F.
Format: Article
Language:English
Subjects:
631/1647/2300
631/449
631/449/447
631/449/447/2311
Agriculture
Base Sequence
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cell culture
Cell differentiation
CRISPR-Associated Protein 9 - metabolism
Deoxyribonucleic acid
DNA
Explants
Gene Editing
Genetic modification
Genome editing
Genomes
Genotypes
Hormones
Life Sciences
Meristem - genetics
Meristems
Mutation - genetics
Nicotiana - genetics
Nicotiana - growth & development
Plant cells
Plant Proteins - metabolism
Plant Shoots - genetics
Plant species
Plants, Genetically Modified
Reagents
Regulators
Seedlings - genetics
Shoots
Soil
Somatic cells
Tissue culture
Transgenes
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Description
Summary:Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, time-consuming, works for only limited species and genotypes, and causes unintended changes to the genome and epigenome. Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem induction. Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants. This induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitted to the next generation. The de novo induction of gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in plant gene editing. Methods to induce edited somatic plant cells to form meristems circumvent tissue culture and enable genome editing of a wider set of plant species.
ISSN:1087-0156
1546-1696
DOI:10.1038/s41587-019-0337-2