Phosphoribosyl pyrophosphate synthetases 2 knockdown inhibits prostate cancer progression by suppressing cell cycle and inducing cell apoptosis

Phosphoribosyl pyrophosphate synthetases 2 (PRPS2) protein function as nucleotide synthesis enzyme that plays vital roles in cancer biology. However, the expression profile and function of PRPS2 in prostate cancer (PCa) remain to be identified. Here we investigated the expression of PRPS2 protein in...

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Bibliographic Details
Published in:Journal of Cancer 2020, Vol.11 (5), p.1027-1037
Main Authors: Qiao, Hui, Tan, Xiao, Lv, Dao-Jun, Xing, Rong-Wei, Shu, Fang-Peng, Zhong, Chuan-Fan, Li, Chun, Zou, Ya-Guang, Mao, Xiang-Ming
Format: Article
Language:English
Subjects:
Androgens
Apoptosis
Biopsy
Biosynthesis
Biotechnology
Cancer therapies
Cell cycle
Cell growth
Chemotherapy
Prostate cancer
Protein expression
Proteins
Research Paper
Tumorigenesis
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Summary:Phosphoribosyl pyrophosphate synthetases 2 (PRPS2) protein function as nucleotide synthesis enzyme that plays vital roles in cancer biology. However, the expression profile and function of PRPS2 in prostate cancer (PCa) remain to be identified. Here we investigated the expression of PRPS2 protein in human PCa and paired normal tissues by immunohistochemistry, meanwhile the regulatory effects on cell proliferation, apoptosis and growth of xenograft tumors in nude mice were evaluated in PCa cells with PRPS2 depletion. Moreover, the signaling pathways were also explored by western blot analysis and quantitative polymerase chain reaction assays. We found that PRPS2 was dramatically upregulated in prostate adenocarcinoma tissues in comparison with normal tissues, and that increased PRPS2 was linked intimately to advanced clinical stage and pT status. Functional experiments showed that knockdown of PRPS2 significantly suppressed cell growth both and . In addition, depletion of PRPS2 induced G phase cell cycle arrest and elevated cell apoptosis. Silencing of PRPS2 resulted in the decreased expression of Bcl‑2 and cyclinD1 and increased levels of Bax, cleavage of caspases‑3, caspases‑9 and PARP. Furthermore, we also detected PRPS2 expression was significantly induced after DHT treatment, which implied the important role of PRPS2 in oncogenesis of PCa. Taken together, our findings elucidated that PRPS2 may be a potential novel candidate for PCa therapy.
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.37401