Effect of Soybean Soluble Polysaccharide on the Formation of Glucono-δ-Lactone-Induced Soybean Protein Isolate Gel

The effect of soybean soluble polysaccharide (SSPS) on the formation of glucono-δ-lactone (GDL)-induced soybean protein isolate (SPI) gel was investigated. Electrophoretic analysis showed the SSPS did not change the electrophoretic behavior of SPI during the formation of SPI gel. However, infrared a...

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Bibliographic Details
Published in:Polymers 2019-12, Vol.11 (12), p.1997
Main Authors: Lan, Qiuyu, Li, Lin, Dong, Hongmin, Wu, Dingtao, Chen, Hong, Lin, Derong, Qin, Wen, Yang, Wenyu, Vasanthan, Thava, Zhang, Qing
Format: Article
Language:English
Subjects:
Coils
Conjugates
Electrophoresis
Fourier transforms
Gluconolactone
Hydrogen bonds
Infrared analysis
Maillard reaction
Polysaccharides
Proteins
Reagents
Rheology
Soy products
Soybeans
Spectrum analysis
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Summary:The effect of soybean soluble polysaccharide (SSPS) on the formation of glucono-δ-lactone (GDL)-induced soybean protein isolate (SPI) gel was investigated. Electrophoretic analysis showed the SSPS did not change the electrophoretic behavior of SPI during the formation of SPI gel. However, infrared analysis indicated the β-sheet content increased, and the contents of random coil and α-helix decreased in both cooked SPI and SPI gel. The SSPS and SPI might conjugate via the Maillard reaction according to the results of grafting degree, color change, and infrared analyses. The main interactions during the formation of SPI gel changed from non-covalent to electrostatic interaction after adding SSPS. Sulfhydryl group content also increased in both cooked SPI and SPI gel. The water-holding capacity and gel strength of SPI gel decreased as the SSPS concentration increased. Larger aggregate holes were observed in the microstructure of SPI gel at higher SSPS concentration. Thus, SSPS could covalently conjugate with SPI and influence the formation of hydrogen bonds, disulfide bonds, and electrostatic interaction among SPI molecules to eventually form a loose gel network.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym11121997