NF-kappa B Signaling-Related Signatures Are Connected with the Mesenchymal Phenotype of Circulating Tumor Cells in Non-Metastatic Breast Cancer

The role of circulating tumor cells (CTCs), tumor microenvironment (TME), and the immune system in the formation of metastasis is evident, yet the details of their interactions remain unknown. This study aimed at exploring the immunotranscriptome of primary tumors associated with the status of CTCs...

Full description

Saved in:
Bibliographic Details
Published in:Cancers 2019-12, Vol.11 (12), p.1961
Main Authors: Popeda, Marta, Stokowy, Tomasz, Bednarz-Knoll, Natalia, Jurek, Anna, Niemira, Magdalena, Bielska, Agnieszka, Kretowski, Adam, Kalinowski, Leszek, Szade, Jolanta, Markiewicz, Aleksandra, Zaczek, Anna J
Format: Article
Language:English
Subjects:
Breast cancer
Gene expression
Genomes
Genotype & phenotype
Immune system
Interferon
Invasiveness
Medical prognosis
Mesenchyme
Metastases
Metastasis
NF-κB protein
Paraffin
Phenotypes
Tumor cells
Tumor microenvironment
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The role of circulating tumor cells (CTCs), tumor microenvironment (TME), and the immune system in the formation of metastasis is evident, yet the details of their interactions remain unknown. This study aimed at exploring the immunotranscriptome of primary tumors associated with the status of CTCs in breast cancer (BCa) patients. The expression of 730 immune-related genes in formalin-fixed paraffin-embedded samples was analyzed using the multigenomic NanoString technology and correlated with the presence and the phenotype of CTCs. Upregulation of 37 genes and downregulation of 1 gene were observed in patients characterized by a mesenchymal phenotype of CTCs when compared to patients with epithelial CTCs. The upregulated genes were involved in NF-kappa B signaling and in the production of type I interferons. The clinical significance of the differentially expressed genes was evaluated using The Cancer Genome Atlas (TCGA) data of a breast invasive carcinoma (BRCA) cohort. Five of the upregulated genes- , , , and -were independent prognostic factors in terms of overall and disease-free survival. To conclude, our data identify a group of genes that are upregulated in BCa patients with mesenchymal CTCs and reveal their prognostic potential, thus indicating that they merit further investigation.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers11121961