Integrated whole liver histologic analysis of the allogeneic islet distribution and characteristics in a nonhuman primate model

The most obvious method to observe transplanted islets in the liver is direct biopsy, but the distribution and location of the best biopsy site in the recipient’s liver are poorly understood. Islets transplanted into the whole liver of five diabetic cynomolgus monkeys that underwent insulin-independ...

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Bibliographic Details
Published in:Scientific reports 2020-01, Vol.10 (1), p.793-793, Article 793
Main Authors: Kim, Geun Soo, Lee, Jong Hyun, Shin, Du Yeon, Lee, Han Sin, Park, Hyojun, Lee, Kyo Won, Yang, Heung-Mo, Kim, Sung Joo, Park, Jae Berm
Format: Article
Language:English
Subjects:
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14/63
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Allografts
Animals
Beta cells
Biopsy
Diabetes mellitus
Diabetes Mellitus, Experimental - pathology
Female
Humanities and Social Sciences
Image Processing, Computer-Assisted
Immunohistochemistry
Insulin
Insulin - metabolism
Islet cells
Islets of Langerhans
Islets of Langerhans Transplantation - methods
Liver
Liver - cytology
Macaca fascicularis
Male
multidisciplinary
Pancreatic islet transplantation
Science
Science (multidisciplinary)
Statistical analysis
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Summary:The most obvious method to observe transplanted islets in the liver is direct biopsy, but the distribution and location of the best biopsy site in the recipient’s liver are poorly understood. Islets transplanted into the whole liver of five diabetic cynomolgus monkeys that underwent insulin-independent survival for an extended period of time after allo-islet transplantation were analyzed for characteristics and distribution tendency. The liver was divided into segments (S1–S8), and immunohistochemistry analysis was performed to estimate the diameter, beta cell area, and islet location. Islets were more distributed in S2 depending on tissue size; however, the number of islets per tissue size was high in S1 and S8. Statistical analysis revealed that the characteristics of islets in S1 and S8 were relatively similar to other segments despite various transplanted islet dosages and survival times. In conclusion, S1, which exhibited high islet density and reflected the overall characteristics of transplanted islets, can be considered to be a reasonable candidate for a liver biopsy site in this monkey model. The findings obtained from the five monkey livers with similar anatomical features to human liver can be used as a reference for monitoring transplanted islets after clinical islet transplantation.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-57701-8