Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13

• Published in: 3 Biotech 2020-02, Vol.10 (2), p.54, Article 54
• Main Authors: Guo, Lei, Wang, Le, Li, Xiangrong, Xu, Xiaowen, Guo, Jiacai, Wang, Xintong, Yang, Weiqin, Xu, Fuxuan, Li, Fuhou
• Format: Article
• Language: English
• Subjects: Agriculture; Aspergillus flavipes; Bioinformatics; Biomaterials; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; Dextrose; Fermentation; Fractional factorial design; Fungi; Inoculum; Optimization; Response surface methodology; Shaking; Short Reports; Sodium chloride; Sodium hydrogen phosphate; Starch; Stem Cells
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-020-2067-z

Questin has favorable applications. Fractional factorial design, Box–Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% Na 2 HPO 4 , pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud’s dextrose medium. Results indicate the potential of A . flavipes HN4-13 in the large-scale production of questin through fermentation.