3D revelation of phenotypic variation, evolutionary allometry, and ancestral states of corolla shape: a case study of clade Corytholoma (subtribe Ligeriinae, family Gesneriaceae)

• Published in: Gigascience 2020-01, Vol.9 (1)
• Main Authors: Hsu, Hao-Chun, Chou, Wen-Chieh, Kuo, Yan-Fu
• Format: Article
• Language: English
• Subjects: Allometry; Biological Evolution; Biological Variation, Population; Centroids; Computed tomography; Curvature; Dilation; Evolution; Flowers; Flowers - anatomy & histology; Image Processing, Computer-Assisted; Imaging, Three-Dimensional; Morphology; Morphometry; Phenotype; Phenotypic variations; Phylogeny; Plant reproduction; Pollination; Quantitative Trait, Heritable; Spatial data
• ISSN: 2047-217X; 2047-217X
• DOI: 10.1093/gigascience/giz155

Abstract Background Quantification of corolla shape variations helps biologists to investigate plant diversity and evolution. 3D images capture the genuine structure and provide comprehensive spatial information. Results This study applied X-ray micro-computed tomography (μCT) to acquire 3D structures of the corollas of clade Corytholoma and extracted a set of 415 3D landmarks from each specimen. By applying the geometric morphometrics (GM) to the landmarks, the first 4 principal components (PCs) in the 3D shape and 3D form analyses, respectively, accounted for 87.86% and 96.34% of the total variance. The centroid sizes of the corollas only accounted for 5.46% of the corolla shape variation, suggesting that the evolutionary allometry was weak. The 4 morphological traits corresponding to the 4 shape PCs were defined as tube curvature, lobe area, tube dilation, and lobe recurvation. Tube curvature and tube dilation were strongly associated with the pollination type and contained phylogenetic signals in clade Corytholoma. The landmarks were further used to reconstruct corolla shapes at the ancestral states. Conclusions With the integration of μCT imaging into GM, the proposed approach boosted the precision in quantifying corolla traits and improved the understanding of the morphological traits corresponding to the pollination type, impact of size on shape variation, and evolution of corolla shape in clade Corytholoma.