Multiphoton microscopy: a personal historical review, with some future predictions

The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are...

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Bibliographic Details
Published in:Journal of biomedical optics 2020-01, Vol.25 (1), p.014511-014511
Main Author: Sheppard, Colin J. R
Format: Article
Language:English
Subjects:
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
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Summary:The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.25.1.014511