Overexpression of HbMBF1a, encoding multiprotein bridging factor 1 from the halophyte Hordeum brevisubulatum, confers salinity tolerance and ABA insensitivity to transgenic Arabidopsis thaliana

• Published in: Plant molecular biology 2020-01, Vol.102 (1-2), p.1-17
• Main Authors: Zhang, Lili, Wang, Yunxiao, Zhang, Qike, Jiang, Ying, Zhang, Haiwen, Li, Ruifen
• Format: Article
• Language: English
• Subjects: Abscisic acid; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biochemistry; Biomedical and Life Sciences; Cotyledon - metabolism; Gene expression; Gene Expression Regulation, Plant; Genes, Plant - genetics; Germination; Greening; Halophytes; Hordeum; Hordeum - genetics; Hordeum - metabolism; Life Sciences; Phenotype; Phenotypes; Phylogeny; Plant growth; Plant Pathology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - growth & development; Plant Sciences; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Salinity; Salinity effects; Salinity tolerance; Salt; Salt tolerance; Salt Tolerance - genetics; Salt Tolerance - physiology; Salt-Tolerant Plants - genetics; Salt-Tolerant Plants - metabolism; Salts - pharmacology; Seed germination; Seedlings; Seedlings - growth & development; Sequence Analysis, DNA; Stress, Physiological - genetics; Trans-Activators - classification; Trans-Activators - genetics; Trans-Activators - metabolism; Transcription; Transcriptome; Transformation, Genetic; Transgenic plants
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-019-00926-7

Key message HbMBF1a was isolated and characterized in H. brevisubulatum , and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana . The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity is an abiotic stress that considerably affects plant growth, yield, and distribution. Hordeum brevisubulatum is a halophyte that evolved to become highly tolerant to salinity. Multiprotein bridging factor 1 (MBF1) is a transcriptional coactivator and an important regulator of stress tolerance. In this study, we isolated and characterized HbMBF1a based on the transcriptome data of H. brevisubulatum grown under saline conditions. We overexpressed HbMBF1a in Arabidopsis thaliana and compared the phenotypes of the transgenic lines and the wild-type in response to stresses. The results indicated that HbMBF1a expression was induced by salt and ABA treatments during the middle and late stages. The overexpression of HbMBF1a in A. thaliana resulted in enhanced salt tolerance and ABA insensitivity. More specifically, the enhanced salt tolerance manifested as the increased seed germination and seedling growth and development. Similarly, under ABA treatments, the cotyledon greening rate and seedling root length were higher in the HbMBF1a -overexpressing lines, suggesting the transgenic plants were better adapted to high exogenous ABA levels. Furthermore, the transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Thus, HbMBF1a is a positive regulator of salt and ABA responses, and the corresponding gene may be useful for producing transgenic plants that are salt tolerant and/or ABA insensitive, with few adverse effects. This study involved a comprehensive analysis of HbMBF1a . The results may provide the basis and insight for the application of MBF1 family genes for developing stress-tolerant crops.