A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves

The transcription factor ZENK is an immediate early gene that has been employed as a surrogate marker to map neuronal activity in the brain. It has been used in a wide variety of species, however, commercially available antibodies have limited immunoreactivity in birds. To address this issue we gene...

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Bibliographic Details
Published in:Scientific reports 2020-01, Vol.10 (1), p.915, Article 915
Main Authors: Nordmann, Gregory Charles, Malkemper, Erich Pascal, Landler, Lukas, Ushakova, Lyubov, Nimpf, Simon, Heinen, Robert, Schuechner, Stefan, Ogris, Egon, Keays, David Anthony
Format: Article
Language:English
Subjects:
13/1
13/51
631/1647/664/1257
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Animals
Antibodies, Monoclonal, Murine-Derived - metabolism
Antigens
Auditory Pathways - physiology
Birds
Birds - immunology
Birds - physiology
Brain - cytology
Brain - physiology
Cloning
Columbidae
Early Growth Response Protein 1 - immunology
Experiments
Fibroblasts
Humanities and Social Sciences
Immunoreactivity
Mice
Monoclonal antibodies
multidisciplinary
Nervous system
Neurons - physiology
Peptides
Proteins
Rocks
Science
Science (multidisciplinary)
Sensory perception
Transcription factors
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Summary:The transcription factor ZENK is an immediate early gene that has been employed as a surrogate marker to map neuronal activity in the brain. It has been used in a wide variety of species, however, commercially available antibodies have limited immunoreactivity in birds. To address this issue we generated a new mouse monoclonal antibody, 7B7-A3, raised against ZENK from the rock pigeon ( Columba livia ). We show that 7B7-A3 labels cl ZENK in both immunoblots and histological stainings with high sensitivity and selectivity for its target. Using a sound stimulation paradigm we demonstrate that 7B7-A3 can detect activity-dependent ZENK expression at key stations of the central auditory pathway of the pigeon. Finally, we compare staining efficiency across three avian species and confirm that 7B7-A3 is compatible with immunohistochemical detection of ZENK in the rock pigeon, zebra finch, and domestic chicken. Taken together, 7B7-A3 represents a useful tool for the avian neuroscience community to map functional activity in the brain.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-57757-6