Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluo...

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Bibliographic Details
Published in:Scientific reports 2020-01, Vol.10 (1), p.1122-1122, Article 1122
Main Authors: Gómez-Varela, Ana I., Stamov, Dimitar R., Miranda, Adelaide, Alves, Rosana, Barata-Antunes, Cláudia, Dambournet, Daphné, Drubin, David G., Paiva, Sandra, De Beule, Pieter A. A.
Format: Article
Language:English
Subjects:
639/624/1107/328/1262
639/624/1107/328/2238
639/766/747
Atomic force microscopy
Epithelial cells
Fluorescence
Fluorescence microscopy
Green fluorescent protein
Humanities and Social Sciences
Microscopes
Microscopy
multidisciplinary
Osteosarcoma
Science
Science (multidisciplinary)
Spatial discrimination
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Summary:Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-57885-z