Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts

• Published in: International journal of molecular sciences 2019-12, Vol.21 (1), p.49
• Main Authors: Li, Lu, Ngo, Hien T T, Hwang, Eunson, Wei, Xuan, Liu, Ying, Liu, Jia, Yi, Tae-Hoo
• Format: Article
• Language: English
• Subjects: Activator protein 1; Adipose Tissue - cytology; Adipose Tissue - metabolism; Aging; Aging (artificial); Antioxidants; Apoptosis; Binding sites; Cell culture; Cell Culture Techniques; Cells, Cultured; Collagen; Culture Media, Conditioned - pharmacology; Cytokines - metabolism; Fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - radiation effects; Gene Expression Regulation - drug effects; Gene Expression Regulation - radiation effects; Growth factors; Heme; Heme oxygenase (decyclizing); Humans; Interleukin 6; Interstitial collagenase; Keratinocytes; Keratinocytes - cytology; Keratinocytes - drug effects; Keratinocytes - radiation effects; Kinases; Matrix metalloproteinase; Matrix Metalloproteinase 1 - metabolism; Matrix metalloproteinases; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Metalloproteinase; NF-κB protein; Organs; Oxygenase; Phosphorylation; Procollagen; Proteins; Radiation; Signal transduction; Signal Transduction - drug effects; Signal Transduction - radiation effects; Skin Aging - drug effects; Stem cells; Transcription factors; Transforming growth factor-b; Ultraviolet Rays - adverse effects; Wound healing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21010049

Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was found that ADSC-CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-κB). In addition, ADSC-CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression of collagen synthesis enhancer gene transforming growth factor-β (TGF-β). The expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging activities of ADSC-CM in cell-based models.