Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean

Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by...

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Bibliographic Details
Published in:Scientific reports 2020-01, Vol.10 (1), p.1356-1356, Article 1356
Main Authors: Kim, Se-Hwan, Jung, ByungHoon, Hong, Soon Gyu, Jung, Kwang-Hwan
Format: Article
Language:English
Subjects:
631/326
631/45
Absorption
Antarctic Regions
Aquatic Organisms - physiology
Cell membranes
Chemical analysis
Humanities and Social Sciences
multidisciplinary
Mutation
Oceans and Seas
Photochemical Processes
Proton Pumps - chemistry
Proton Pumps - genetics
Proton Pumps - metabolism
Protons
Rhodopsin
Rhodopsins, Microbial - chemistry
Rhodopsins, Microbial - genetics
Rhodopsins, Microbial - metabolism
Science
Science (multidisciplinary)
Seawater
Spectrum Analysis
Temperature
Water analysis
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Summary:Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by PCR from the seawater sample near King George Island, Antarctica. One was the same sequence as the first reported GPR (Green-light absorbing PR) from Monterey Bay. Another named HSG119 was a newly discovered sequence which shows high sequence similarity with BPR (Blue-light absorbing PR). HSG119 has an absorption maximum at 493 nm with broader spectrum at pH7.0 and it can pump protons out of the cell membrane. Interestingly, it showed a similar temperature dependence to GPR(Y200N) that isolated near the North pole.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-58023-5