The expression of the long NEAT1_2 isoform is associated with human epidermal growth factor receptor 2-positive breast cancers

• Published in: Scientific reports 2020-01, Vol.10 (1), p.1277-1277, Article 1277
• Main Authors: Knutsen, Erik, Lellahi, Seyed Mohammad, Aure, Miriam Ragle, Nord, Silje, Fismen, Silje, Larsen, Kenneth Bowitz, Gabriel, Marta Tellez, Hedberg, Annica, Bjørklund, Sunniva Stordal, Bofin, Anna Mary, Mælandsmo, Gunhild Mari, Sørlie, Therese, Mortensen, Elin Synnøve, Perander, Maria
• Format: Article
• Language: English
• Subjects: 13; 13/106; 14; 14/32; 38; 38/61; 631/67/1347; 692/4028/67/1347; Breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Epidermal growth factor; ErbB-2 protein; Female; Gene expression; Gene Expression Regulation, Neoplastic; Gene regulation; Humanities and Social Sciences; Humans; Isoforms; Lactation; MCF-7 Cells; Medical disciplines: 700; Medisinske Fag: 700; multidisciplinary; Non-coding RNA; RNA, Long Noncoding - biosynthesis; RNA, Neoplasm - biosynthesis; Science; Science (multidisciplinary); VDP
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-57759-4

The long non-coding RNA NEAT1 locus is transcribed into two overlapping isoforms, NEAT1_1 and NEAT1_2 , of which the latter is essential for the assembly of nuclear paraspeckles. NEAT1 is abnormally expressed in a wide variety of human cancers. Emerging evidence suggests that the two isoforms have distinct functions in gene expression regulation, and recently it was shown that NEAT1_2 , but not NEAT1_1 , expression predicts poor clinical outcome in cancer. Here, we report that NEAT1_2 expression correlates with HER2-positive breast cancers and high-grade disease. We provide evidence that NEAT1_1 and NEAT1_2 have distinct expression pattern among different intrinsic breast cancer subtypes. Finally, we show that NEAT1_2 expression and paraspeckle formation increase upon lactation in humans, confirming what has previously been demonstrated in mice.