Success and efficiency of cell seeding in Avian Tendon Xenografts – A promising alternative for tendon and ligament reconstruction

AbstractDecellularized tendon xenografts offer a promising alternative for reconstruction by using ubiquitously available material. This study compares static and centrifugal seeding of avian tendon scaffolds with NIH 3T3 fibroblasts. Incorporation of viable cells was achievable with both techniques...

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Bibliographic Details
Published in:Journal of orthopaedics 2020-03, Vol.18, p.155-161
Main Authors: Thönnes, Simon, Shelton, Peter, Bracey, Daniel N, Van Dyke, Mark, Whitlock, Patrick, Smith, Thomas L, Moghaddam, Arash, Tuohy, Christopher
Format: Article
Language:English
Subjects:
Cell adhesion
Cell seeding efficiency
Confocal laser microscopy
Orthopedics
Tendon scaffold
Tissue engineering
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Summary:AbstractDecellularized tendon xenografts offer a promising alternative for reconstruction by using ubiquitously available material. This study compares static and centrifugal seeding of avian tendon scaffolds with NIH 3T3 fibroblasts. Incorporation of viable cells was achievable with both techniques, represented by DNA content. Proliferation rate and viability assay showed neither damage by centrifugal force nor superiority of the technique. Cell proliferation after 10 days of culture demonstrated that the scaffold did not hinder 3-D culturing. Confocal laser microscopy revealed structural details as formation of focal adhesions, to provide deeper insight into the process of cell attachment and growth in xenografts.
ISSN:0972-978X
0972-978X
DOI:10.1016/j.jor.2019.09.010