Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification

Increasing interest is aroused by traditional Chinese medicine (TCM) treatment of chronic hepatitis B (CHB) based on specific TCM syndrome. As the most common CHB syndromes, spleen-stomach dampness-heat (SSDH) syndrome and liver-gallbladder dampness-heat (LGDH) syndrome are still apt to be confused...

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Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-11
Main Authors: Ye, Hua, Li, Baixue, Feng, Quansheng, Liu, Xiyang, Su, Yue, Li, Guiyu, Yan, Di, Wang, Dong, Wan, Tingjun, Jiang, Cen, Wen, Li, Rong, Lijun
Format: Article
Language:English
Subjects:
Analysis
Bioinformatics
Biomarkers
Care and treatment
Chinese medicine
Diagnosis
DNA microarrays
Ethylenediaminetetraacetic acid
Evidence-based medicine
Gallbladder
Genomes
Heat
Hepatitis
Hepatitis B
Liver
Liver cancer
Medical diagnosis
Medical research
Medicine, Chinese
Medicine, Experimental
MicroRNA
MicroRNAs
miRNA
Software
Spleen
Traditional Chinese medicine
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Summary:Increasing interest is aroused by traditional Chinese medicine (TCM) treatment of chronic hepatitis B (CHB) based on specific TCM syndrome. As the most common CHB syndromes, spleen-stomach dampness-heat (SSDH) syndrome and liver-gallbladder dampness-heat (LGDH) syndrome are still apt to be confused in TCM diagnosis, greatly hindering the stable exertion of TCM effectiveness. It is urgently needed to provide objective and biological evidences for differentiation and identification of the two significant syndromes. In this study, microRNA (miRNA) microarray analyses coupled with bioinformatics were employed for comparative miRNA profiling of SSDH and LGDH patients. It was found that the two syndromes had both the same and different significantly differentially expressed miRNAs (SDE-miRNAs). Commonness and specificity were also both found between their SDE-miRNA-based bioinformatics analyses, including Hierarchical Clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and miRNA-GO/pathway networks. Furthermore, syndrome-specific SDE-miRNAs were identified as the potential biomarkers, including hsa-miR-1273g-3p and hsa-miR-4419b for SSDH as well as hsa-miR-129-1-3p and hsa-miR-129-2-3p for LGDH. All these laid biological and clinical bases for classification and diagnosis of the two significant CHB dampness-heat syndromes including SSDH and LGDH, providing more opportunities for better application of TCM efficacy and superiority in CHB treatment.
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/7234893