Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

• Published in: Plant biotechnology journal 2020-03, Vol.18 (3), p.614-630
• Main Authors: Chen, Lei, Zhao, Jiqiang, Song, Jiancheng, Jameson, Paula E.
• Format: Article
• Language: English
• Subjects: Agricultural production; Barley; Cereal crops; Cereals; CKX; Corn; Crop yield; cytokinin; cytokinin oxidase/dehydrogenase; Cytokinins; Dehydrogenase; Dehydrogenases; Enzymes; Gene expression; Genomes; Genomics; Grain; mineral nutrition; Mutation; Nutrients; Nutrition; Plant growth; Plant hormones; Review; Rice; root growth; Senescence; Signal transduction; TILLING; Wheat; whole‐exome sequencing; yield; Zinc in the body
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/pbi.13305

Summary The plant hormone group, the cytokinins, is implicated in both qualitative and quantitative components of yield. Cytokinins have opposing actions in shoot and root growth—actions shown to involve cytokinin dehydrogenase (CKX), the enzyme that inactivates cytokinin. We revise and provide unambiguous names for the CKX gene family members in wheat, based on the most recently released wheat genome database, IWGSC RefSeq v1.0 & v2.0. We review expression data of CKX gene family members in wheat, revealing tissue‐specific gene family member expression as well as sub‐genome‐specific expression. Manipulation of CKX in cereals shows clear impacts on yield, root growth and orientation, and Zn nutrition, but this also emphasizes the necessity to unlink promotive effects on grain yield from negative effects of cytokinin on root growth and uptake of mineral nutrients, particularly Zn and Fe. Wheat is the most widely grown cereal crop globally, yet is under‐research compared with rice and maize. We highlight gaps in our knowledge of the involvement of CKX for wheat. We also highlight the necessity for accurate analysis of endogenous cytokinins, acknowledging why this is challenging, and provide examples where inadequate analyses of endogenous cytokinins have led to unjustified conclusions. We acknowledge that the allohexaploid nature of bread wheat poses challenges in terms of uncovering useful mutations. However, we predict TILLING followed by whole‐exome sequencing will uncover informative mutations and we indicate the potential for stacking mutations within the three genomes to modify yield components. We model a wheat ideotype based on CKX manipulation.