Optical coherence tomography-guided dynamic focusing for combined optical and mechanical scanning multimodal photoacoustic microscopy

To achieve fast imaging and large field of view (FOV), we improved our multimodal imaging system, which integrated optical resolution photoacoustic microscopy, optical coherence tomography (OCT), and confocal fluorescence microscopy in one platform, by combining optical scanning with mechanical scan...

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Bibliographic Details
Published in:Journal of biomedical optics 2019-12, Vol.24 (12), p.121906-121906
Main Authors: Dadkhah, Arash, Jiao, Shuliang
Format: Article
Language:English
Subjects:
Biological properties
Biological samples
Dynamic focusing
Fluorescence
Fluorescence microscopy
Imaging
Lasers
Light
Microscopy
Optical Coherence Tomography
Photoacoustic microscopy
Scanning
Special Section Celebrating the Exponential Growth of Biomedical Optoacoustic/Photoacoustic Imaging
Tissues
Tomography
Ultrasonic transducers
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Summary:To achieve fast imaging and large field of view (FOV), we improved our multimodal imaging system, which integrated optical resolution photoacoustic microscopy, optical coherence tomography (OCT), and confocal fluorescence microscopy in one platform, by combining optical scanning with mechanical scanning. To ensure good focusing of the objective lens over all the imaged area, we employed OCT-guided dynamic focusing. Different from our previous point-by-point dynamic focusing, we employed an area-by-area focusing adjustment strategy, in which each fast optical scanning area has a fixed focusing depth. We have demonstrated the performance of the system by imaging biological samples ex vivo (plant leaf) and in vivo (mouse ear). The system has achieved uniform resolution in an FOV of 10  mm  ×  10  mm with an imaging time of about 5 min.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.24.12.121906