Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

• Published in: Scientific reports 2020-02, Vol.10 (1), p.2109, Article 2109
• Main Authors: Patel, S. B., Attar, U. A., Sakate, D. M., Ghane, S. G.
• Format: Article
• Language: English
• Subjects: 631/449; 631/45; alpha-Amylases - antagonists & inhibitors; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antitumor activity; Breast; Cancer; Chloroform; Colon; Colon cancer; Cucurbitaceae - chemistry; Cucurbitacins - isolation & purification; Cucurbitacins - pharmacology; Diabetes mellitus; Fruits; Glycoside Hydrolase Inhibitors - isolation & purification; Glycoside Hydrolase Inhibitors - pharmacology; HT29 Cells - drug effects; Humanities and Social Sciences; Humans; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; MCF-7 Cells - drug effects; Medicinal plants; multidisciplinary; Oxidative stress; Particle size; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Science; Science (multidisciplinary); Steam; Tumor cell lines; α-Amylase; α-Glucosidase
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-58924-5

Diplocyclos palmatus (L.) C. Jeffrey is an important medicinal plant used in several reproductive medicines. It serves as a wide source of tetracyclic triterpens called cucurbitacins. Response surface methodology (RSM) with Box-Behnken design (BBD) was studied to optimize the production of cucurbitacins. RSM put forth the ideal conditions such as 1:30 SS ratio (g/mL), 80 rpm (mixing extraction speed), 150 µm mean particle size, 30 min extraction time and 50  ° C using chloroform in continuous shaking extraction (CSE) and showed the highest cucurbitacin I (CUI) content (2.345 ± 0.1686 mg/g DW). Similarly, the highest yield of cucurbitacin B (CUB) (1.584 ± 0.15 mg/g DW) was recorded at ideal conditions (1:40 g/mL SS ratio and 60 min time and others similar to CUI). Among the tested extraction methods, the highest CUI, CUB, and CUI + B yield (1.437 ± 0.03, 0.782 ± 0.10, 2.17 ± 0.35 mg/g DW, respectively) as well as promising DPPH radical scavenging activity (25.06 ± 0.1 µgAAE/g DW) were recorded from the SBAE (steam bath assisted extraction). In addition, MAE and UAE revealed the highest inhibition of α-amylase (68.68%) and α-glucosidase (56.27%) enzymes, respectively. Fruit extracts showed potent anticancer activity against breast (MCF-7) and colon (HT-29) cancer cell lines (LC 50 – 44.27 and 46.88 µg/mL, respectively). Our study proved that SS ratio, particle size and temperature were the most positively influencing variables and served to be the most efficient for the highest recovery of CUI and CUB. Based on the present study, the fruits of D. palmatus were revealed as a potent antioxidant, anti-diabetic and anticancer bio-resource that could be explored further to develop novel drug to manage diabetes, cancer and oxidative stress related disorders.