Pressure sensing through Piezo channels controls whether cells migrate with blebs or pseudopods

Blebs and pseudopods can both power cell migration, with blebs often favored in tissues, where cells encounter increased mechanical resistance. To investigate how migrating cells detect and respond to mechanical forces, we used a “cell squasher” to apply uniaxial pressure to Dictyostelium cells chem...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2020-02, Vol.117 (5), p.2506-2512
Main Authors: Srivastava, Nishit, Traynor, David, Piel, Matthieu, Kabla, Alexandre J., Kay, Robert R.
Format: Article
Language:English
Subjects:
Actin
Biological Sciences
Biomechanical Phenomena
Calcium
Calcium (extracellular)
Calcium influx
Cell adhesion & migration
Cell Behavior
Cell migration
Cell Movement
Cellular Biology
Chemotaxis
Cytoplasm
Cytoplasm - chemistry
Cytoplasm - genetics
Cytoplasm - metabolism
Cytoskeleton
Dictyostelium - chemistry
Dictyostelium - cytology
Dictyostelium - genetics
Dictyostelium - metabolism
Ion Channels - genetics
Ion Channels - metabolism
Life Sciences
Mechanotransduction, Cellular
Myosin
Myosin Type II - genetics
Myosin Type II - metabolism
PNAS Plus
Pressure
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Pseudopodia - genetics
Pseudopodia - metabolism
Subcellular Processes
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Summary:Blebs and pseudopods can both power cell migration, with blebs often favored in tissues, where cells encounter increased mechanical resistance. To investigate how migrating cells detect and respond to mechanical forces, we used a “cell squasher” to apply uniaxial pressure to Dictyostelium cells chemotaxing under soft agarose. As little as 100 Pa causes a rapid (
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1905730117