Optimization of Extraction Conditions of Phytochemical Compounds and Anti-Gout Activity of Euphorbia hirta L. (Ara Tanah) Using Response Surface Methodology and Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis

Gout is a common disease affected most of the people due to the elevation of uric acid in the blood. Flavonoid and phenolic compounds are reported to exert the anti-gout activity of medicinal plants. Hence, this study aimed at optimizing the extraction conditions of phenolic and flavonoid compounds...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-13
Main Authors: Endrini, Susi, Abdullah, Norazlin, Abu Bakar, Mohd Fadzelly, Abu Bakar, Fazleen Izzany, Fatmawati, Sri
Format: Article
Language:English
Subjects:
Asthma
Caffeic acid
Chromatography
Ellagic acid
Enzymes
Euphorbia hirta
Evidence-based medicine
Flavonoids
Functional foods & nutraceuticals
Gout
Liquid chromatography
Mass spectroscopy
Medical research
Medicinal plants
Optimization
Phenolic compounds
Phytochemicals
Potassium
Quercetin
Rheumatism
Solvents
Uric acid
Xanthine oxidase
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Summary:Gout is a common disease affected most of the people due to the elevation of uric acid in the blood. Flavonoid and phenolic compounds are reported to exert the anti-gout activity of medicinal plants. Hence, this study aimed at optimizing the extraction conditions of phenolic and flavonoid compounds as well as the anti-gout (xanthine oxidase inhibitory activity) in vitro of Euphorbia hirta using response surface methodology (RSM). The plant part used was the whole plant excluding roots. The effects of three independent variables (extraction time, X1; extraction temperature, X2; and solid-to-liquid ratio, X3) on three response variables (total flavonoid content, Y1; total phenolic content, Y2; and xanthine oxidase inhibitory activity, Y3) were determined using central composite design (CCD) while phytochemical profiling of the extracts was determined by liquid chromatography-mass spectrometry (LC-MS). Quadratic models produced a satisfactory fitting of the experimental data with regard to total flavonoid content (r2 = 0.9407, p
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/4501261