In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Abstract The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2009-03, Vol.33 (3), p.230-236
Main Authors: Ammons, Mary Cloud B, Ward, Loren S, Fisher, Steve T, Wolcott, Randall D, James, Garth A
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biofilm
Biofilms - drug effects
Biological and medical sciences
Cell Membrane Permeability
Drug Synergism
Humans
Infectious Disease
Lactoferrin
Lactoferrin - pharmacology
Medical sciences
Microbial Viability
Pharmacology. Drug treatments
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - microbiology
Wound Infection - microbiology
Xylitol
Xylitol - pharmacology
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Summary:Abstract The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in vitro evidence to support the use of novel antimicrobials in the treatment of Pseudomonas aeruginosa biofilm. An in vitro biofilm model with a clinical isolate of P. aeruginosa was subjected to treatment with either lactoferrin or xylitol alone or in combination. Combined lactoferrin and xylitol treatment disrupted the structure of the P. aeruginosa biofilm and resulted in a >2log reduction in viability. In situ analysis indicated that while xylitol treatment appeared to disrupt the biofilm structure, lactoferrin treatment resulted in a greater than two-fold increase in the number of permeabilised bacterial cells. The findings presented here indicated that combined treatment with lactoferrin and xylitol significantly decreases the viability of established P. aeruginosa biofilms in vitro and that the antimicrobial mechanism of this treatment includes both biofilm structural disruption and permeablisation of bacterial membranes.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2008.08.013