The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species

Directing the flow of protein traffic is a critical task faced by all cellular organisms. In Gram-negative bacteria, this traffic includes lipoproteins. Lipoproteins are synthesized as precursors in the cytoplasm and receive their acyl modifications upon export across the inner membrane. The third a...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2018-12, Vol.164 (12), p.1547-1556
Main Authors: Gwin, Celena M, Prakash, Natalia, Christian Belisario, J, Haider, Lubaina, Rosen, Marlene L, Martinez, Luis R, Rigel, Nathan W
Format: Article
Language:English
Subjects:
Acinetobacter - enzymology
Acinetobacter - genetics
Acinetobacter - growth & development
Acinetobacter - metabolism
Acylation
Acyltransferases - genetics
Acyltransferases - metabolism
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cell Membrane Permeability
Gene Expression Regulation, Bacterial
Lipoproteins - metabolism
Loss of Function Mutation
Microbial Sensitivity Tests
Microbial Viability - drug effects
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Summary:Directing the flow of protein traffic is a critical task faced by all cellular organisms. In Gram-negative bacteria, this traffic includes lipoproteins. Lipoproteins are synthesized as precursors in the cytoplasm and receive their acyl modifications upon export across the inner membrane. The third and final acyl chain is added by Lnt, which until recently was thought to be essential in all Gram-negatives. In this report, we show that Acinetobacter species can also tolerate a complete loss-of-function mutation in lnt. Absence of a fully functional Lnt impairs modification of lipoproteins, increases outer membrane permeability and susceptibility to antibiotics, and alters normal cellular morphology. In addition, we show that loss of lnt triggers a global transcriptional response to this added cellular stress. Taken together, our findings provide new insights on and support the growing revisions to the Gram-negative lipoprotein biogenesis paradigm.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.000726