Genome and Karyotype Reorganization after Whole Genome Duplication in Free-Living Flatworms of the Genus Macrostomum

The genus represents a diverse group of rhabditophoran flatworms with >200 species occurring around the world. Earlier we uncovered karyotype instability linked to hidden polyploidy in both (2 = 8) and its sibling species (2 = 10), prompting interest in the karyotype organization of close relativ...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-01, Vol.21 (2), p.680
Main Authors: Zadesenets, Kira S, Jetybayev, Ilyas Y, Schärer, Lukas, Rubtsov, Nikolay B
Format: Article
Language:English
Subjects:
Aneuploidy
Animal models
Animals
Biological Evolution
Chromosomes
Cytogenetics
Deoxyribonucleic acid
DNA
DNA probes
Evolution
Flatworms
Fluorescence
Fluorescence in situ hybridization
Gene Duplication
Genes
Genome, Helminth - genetics
Genomes
Hybridization
In Situ Hybridization, Fluorescence
Karyotype
Karyotypes
Laboratories
Macrostomum
Mutation
Platyhelminths - genetics
Polyploidy
Reproduction (copying)
Reptiles & amphibians
Stability
Worms
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Summary:The genus represents a diverse group of rhabditophoran flatworms with >200 species occurring around the world. Earlier we uncovered karyotype instability linked to hidden polyploidy in both (2 = 8) and its sibling species (2 = 10), prompting interest in the karyotype organization of close relatives. In this study, we investigated chromosome organization in two recently described and closely related species, and , and explored karyotype instability in laboratory lines and cultures of (DV1/10, 2 = 10) and in more detail. We revealed that three of the four studied species are characterized by karyotype instability, while showed a stable 2 = 6 karyotype. Next, we performed comparative cytogenetics of these species using fluorescent in situ hybridization (FISH) with a set of DNA probes (including microdissected DNA probes generated from chromosomes, rDNA, and telomeric DNA). To explore the chromosome organization of the unusual 2 = 9 karyotype discovered in , we then generated chromosome-specific DNA probes for all chromosomes of this species. Similar to and , our findings suggest that arose via whole genome duplication (WGD) followed by considerable chromosome reshuffling. We discuss possible evolutionary scenarios for the emergence and reorganization of the karyotypes of these species and consider their suitability as promising animal models for studying the mechanisms and regularities of karyotype and genome evolution after a recent WGD.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21020680