Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders

Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffin...

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Bibliographic Details
Published in:Materials 2020-01, Vol.13 (2), p.447
Main Authors: Olshinka, Asaf, Ad-El, Dean, Didkovski, Elena, Weiss, Shirel, Ankri, Rinat, Goldenberg-Cohen, Nitza, Fixler, Dror
Format: Article
Language:English
Subjects:
Antibodies
Borders
Cancer therapies
Diffusion
Disease
Extinction
Gold
Growth factors
Hyperspectral imaging
Identification methods
Kinases
Ligands
Medical prognosis
Metastasis
Methods
Microscopy
Monoclonal antibodies
Nanoparticles
Nanotechnology
Optical properties
Radiation
Reflection
Sensitivity
Skin cancer
Spectrum analysis
Tumors
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Summary:Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free ( < 0.05, -test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma13020447