Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm

Despite the many advantages of poly (methyl methacrylate) (PMMA) as a dental polymer, its antifungal and antibacterial effects remain limited. Here, phytoncide was incorporated into PMMA to inhibit fungal and biofilm accumulation without impairing the basic and biological properties of PMMA. A varia...

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Bibliographic Details
Published in:Materials 2020-01, Vol.13 (2), p.371
Main Authors: Lee, Myung-Jin, Kim, Min-Ji, Oh, Sang-Hwan, Kwon, Jae-Sung
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial agents
Biocompatibility
Biofilms
Biological properties
Cytotoxicity
Dental materials
Dentistry
Dentures
Evaluation
Flexural strength
Fungi
Fungicides
Gloss
Gram-positive bacteria
Material properties
Mechanical properties
Microhardness
Microorganisms
Phenols
Polymers
Polymethyl methacrylate
Prostheses
Saliva
Thickness
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Summary:Despite the many advantages of poly (methyl methacrylate) (PMMA) as a dental polymer, its antifungal and antibacterial effects remain limited. Here, phytoncide was incorporated into PMMA to inhibit fungal and biofilm accumulation without impairing the basic and biological properties of PMMA. A variable amount of phytoncide (0 wt % to 5 wt %) was incorporated into PMMA, and the basic material properties of microhardness, flexural strength and gloss were evaluated. In addition, cell viability was confirmed by MTT assay. This MTT assay measures cell viability via metabolic activity, and the color intensity of the formazan correlates viable cells. The fungal adhesion and viability on the PMMA surfaces were evaluated using (a pathogenic yeast). Finally, the thickness of saliva-derived biofilm was estimated. The flexural strength of PMMA decreased with increasing phytoncide contents, whereas there were no significant differences in the microhardness and gloss ( > 0.05) and the cell viability ( > 0.05) between the control and the phytoncide-incorporated PMMA samples. The amounts of adherent colony-forming unit (CFU) counts, and saliva-derived biofilm thickness were significantly lower in the phytoncide-incorporated PMMA compared to the control ( < 0.05). Hence, it was concluded that the incorporation of appropriate amounts of phytoncide in PMMA demonstrated antifungal effects while maintaining the properties, which could be a possible use in dentistry application such as denture base resin.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma13020371