Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells

Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to...

Full description

Saved in:
Bibliographic Details
Published in:Cancers 2019-12, Vol.12 (1), p.82
Main Authors: Hassan, Ghmkin, Afify, Said M, Nair, Neha, Kumon, Kazuki, Osman, Amira, Du, Juan, Mansour, Hager, Abu Quora, Hagar A, Nawara, Hend M, Satoh, Ayano, Zahra, Maram H, Okada, Nobuhiro, Seno, Akimasa, Seno, Masaharu
Format: Article
Language:English
Subjects:
Adherent cells
Angiogenesis
Bone marrow
Busulfan
Cancer
CD34 antigen
Cell differentiation
Chemokines
Cytology
Fibroblasts
Flow cytometry
Homing behavior
Interleukin 3
Leukemia
Leukocytes
Macrophages
Metastasis
Morphology
Phenotypes
Pluripotency
Stem cell transplantation
Stem cells
Tumor microenvironment
Tumorigenesis
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright-Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers12010082