Serotonergic afferents from the dorsal raphe decrease the excitability of pyramidal neurons in the anterior piriform cortex

The olfactory system receives extensive serotonergic inputs from the dorsal raphe, a nucleus involved in control of behavior, regulation of mood, and modulation of sensory processing. Although many studies have investigated how serotonin modulates the olfactory bulb, few have focused on the anterior...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2020-02, Vol.117 (6), p.3239-3247
Main Authors: Wang, Dejuan, Wang, Xiaojie, Liu, Penglai, Jing, Siqi, Du, Han, Zhang, Lingzhi, Jia, Fan, Li, Anan
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Calcium
Calcium channels
Cortex (piriform)
Dorsal Raphe Nucleus - metabolism
Excitability
Information processing
Mice
Mice, Inbred C57BL
Mice, Transgenic
Modulation
Mood
Neurons
Odor intensity
Odorants
Odors
Olfactory bulb
Olfactory Bulb - physiology
Olfactory discrimination learning
Olfactory system
Optogenetics
Phospholipase
Phospholipase C
Photometry
Piriform Cortex - cytology
Piriform Cortex - metabolism
Potassium
Potassium channels
Pyramidal cells
Pyramidal Cells - metabolism
Receptors
Sensory evaluation
Sensory integration
Serotonergic Neurons - metabolism
Serotonin
Serotonin - genetics
Serotonin - metabolism
Serotonin S2 receptors
Smell
Somatosensory cortex
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Summary:The olfactory system receives extensive serotonergic inputs from the dorsal raphe, a nucleus involved in control of behavior, regulation of mood, and modulation of sensory processing. Although many studies have investigated how serotonin modulates the olfactory bulb, few have focused on the anterior piriform cortex (aPC), a region important for olfactory learning and encoding of odor identity and intensity. Specifically, the mechanism and functional significance of serotonergic modulation of the aPC remain largely unknown. Here we used pharmacologic, optogenetic, and fiber photometry techniques to examine the serotonergic modulation of neural activity in the aPC in vitro and in vivo. We found that serotonin (5-HT) reduces the excitability of pyramidal neurons directly via 5-HT2C receptors, phospholipase C, and calcium-activated potassium (BK) channels. Furthermore, endogenous serotonin attenuates odor-evoked calcium responses in aPC pyramidal neurons. These findings identify the mechanism underlying serotonergic modulation of the aPC and shed light on its potential role.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1913922117