Complementary molecular methods reveal comprehensive phylogenetic diversity integrating inconspicuous lineages of early-diverged wood-decaying mushrooms

Among terrestrial microorganisms, mushroom-forming fungi have been relatively well investigated, however the inconspicuous strains may be overlooked by conventional visual investigations causing underestimation of their phylogenetic diversity. Herein, we sought to obtain a comprehensive phylogenetic...

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Bibliographic Details
Published in:Scientific reports 2020-02, Vol.10 (1), p.3057-3057, Article 3057
Main Authors: Shirouzu, Takashi, Matsuoka, Shunsuke, Doi, Hideyuki, Nagata, Nobuaki, Ushio, Masayuki, Hosaka, Kentaro
Format: Article
Language:English
Subjects:
45/22
45/23
45/77
631/158/855
631/326/193/2539
Agaricales - classification
Base Sequence
Biodiversity
Confidence Intervals
Decay fungi
DNA Barcoding, Taxonomic - methods
Environmental DNA
Forests
Fruit bodies
Humanities and Social Sciences
Linear Models
multidisciplinary
Mycelia
Phylogenetics
Phylogeny
Science
Science (multidisciplinary)
Seasons
Strains (organisms)
Wood - microbiology
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Summary:Among terrestrial microorganisms, mushroom-forming fungi have been relatively well investigated, however the inconspicuous strains may be overlooked by conventional visual investigations causing underestimation of their phylogenetic diversity. Herein, we sought to obtain a comprehensive phylogenetic diversity profile for the early-diverging wood-decaying mushrooms Dacrymycetes, using an approach that combines fruiting-body collection, culture isolation, and environmental DNA (eDNA) metabarcoding of decaying branches. Among the 28 operational taxonomic units (OTUs) detected during a three-year investigation, 10 each were from fruiting bodies and cultured mycelia and 27 were detected as eDNA sequences. eDNA metabarcoding revealed various lineages across the Dacrymycetes phylogeny. Alternatively, fruiting-body and culture surveys uncovered only ~50% of the OTUs detected through eDNA metabarcoding, suggesting that several inconspicuous or difficult-to-isolate strains are latent in the environment. Further, eDNA and culture surveys revealed early-diverging clades that were not identified in the fruiting-body survey. Thus, eDNA and culture-based techniques can uncover inconspicuous yet phylogenetically important mushroom lineages that may otherwise be overlooked via typical visual investigations.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-59620-0