Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica) using ISSR, SCoT and morphological markers

Investigation of genetic diversity is essential for the selection of parents for crop breeding and conservation of genetic resources. To estimate the genetic variability and population structure in the midst of 45 accessions of sponge gourd brought together from different geographical areas of India...

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Bibliographic Details
Published in:Physiology and molecular biology of plants 2020-01, Vol.26 (1), p.119-131
Main Authors: Tyagi, Ruchi, Sharma, Vinay, Sureja, Amish Kumar, Das Munshi, Anilabh, Arya, Lalit, Saha, Dipnarayan, Verma, Manjusha
Format: Article
Language:English
Subjects:
Bayesian analysis
Bayesian theory
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Breeding
Cell Biology
fruit characteristics
Gene polymorphism
genetic background
genetic correlation
Genetic diversity
Genetic markers
Genetic resources
Genetic variability
genetic variation
Gourds
India
Life Sciences
Luffa aegyptiaca
Luffa cylindrica
Markers
microsatellite repeats
Morphology
parents
Plant breeding
Plant Physiology
Plant Sciences
Polymorphism
Population genetics
Population structure
principal component analysis
Principal components analysis
Research Article
Resource conservation
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Summary:Investigation of genetic diversity is essential for the selection of parents for crop breeding and conservation of genetic resources. To estimate the genetic variability and population structure in the midst of 45 accessions of sponge gourd brought together from different geographical areas of India, morphological traits and two molecular markers, ISSR and SCoT markers were compared. Principal components analysis of 20 morphological traits showed 72.70% variability and significant positive correlations between fruit traits. All three marker techniques clustered all accessions into two groups with few outgroups. High level of polymorphism was observed among ISSR (74.6%) and SCoT (71.5%) primers. The Bayesian model revealed the hidden grouping and showed admixture type of population. The diversity pattern is influenced by genetic marker used, as different molecular markers have different polymorphism evaluation efficiency. This study can be helpful in amplifying the genetic base and selection of specific traits for breeding. Thus, ISSR and SCoT markers are potential marker for identification in sponge gourd and provide valuable data on its genetic correlation and structure.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-019-00723-y