A Standard Numbering Scheme for Class C β-Lactamases

• Published in: Antimicrobial agents and chemotherapy 2020-02, Vol.64 (3)
• Main Authors: Mack, Andrew R, Barnes, Melissa D, Taracila, Magdalena A, Hujer, Andrea M, Hujer, Kristine M, Cabot, Gabriel, Feldgarden, Michael, Haft, Daniel H, Klimke, William, van den Akker, Focco, Vila, Alejandro J, Smania, Andrea, Haider, Shozeb, Papp-Wallace, Krisztina M, Bradford, Patricia A, Rossolini, Gian Maria, Docquier, Jean-Denis, Frère, Jean-Marie, Galleni, Moreno, Hanson, Nancy D, Oliver, Antonio, Plésiat, Patrick, Poirel, Laurent, Nordmann, Patrice, Palzkill, Timothy G, Jacoby, George A, Bush, Karen, Bonomo, Robert A
• Format: Article
• Language: English
• Subjects: Amino acid numbering; Amino Acid Sequence; AmpC; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - antagonists & inhibitors; Bacterial Proteins - classification; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-Lactam Resistance - genetics; beta-Lactamase Inhibitors - chemistry; beta-Lactamase Inhibitors - pharmacology; Beta-lactamases; beta-Lactamases - classification; beta-Lactamases - genetics; beta-Lactamases - metabolism; beta-Lactams - chemistry; beta-Lactams - pharmacology; Class C beta-lactamase; Conserved residue; Editor's Pick; Gene Expression; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - enzymology; Gram-Negative Bacteria - genetics; Gram-Positive Bacteria - drug effects; Gram-Positive Bacteria - enzymology; Gram-Positive Bacteria - genetics; International Cooperation; Life Sciences; Mechanisms of Resistance; Microbiologie; Microbiology; Microbiology and Parasitology; Mutation; Nomenclature; Protein Structure, Secondary; Sciences du vivant; Sequence Alignment; Sequence Homology, Amino Acid; Structure-activity relationships; Terminology as Topic
• ISSN: 0066-4804; 1098-6596; 1098-6596
• DOI: 10.1128/AAC.01841-19

Unlike for classes A and B, a standardized amino acid numbering scheme has not been proposed for the class C (AmpC) β-lactamases, which complicates communication in the field. Here, we propose a scheme developed through a collaborative approach that considers both sequence and structure, preserves traditional numbering of catalytically important residues (Ser , Lys , Tyr , and Lys ), is adaptable to new variants or enzymes yet to be discovered and includes a variation for genetic and epidemiological applications.