Effect of miR-205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism

This study explored the target of miR-205 and the effect of miR-205 on the proliferation and migration regulating its target in thyroid cancer cells (TC). Twenty-five pairs of TC and adjacent tissues were collected after surgical resection. Real-time fluorescence quantitative PCR (qRT-PCR) was used...

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Bibliographic Details
Published in:Oncology letters 2020-03, Vol.19 (3), p.2568-2574
Main Authors: Wang, Xin, Zhang, Haomin, Jiao, Kai, Zhao, Chunyang, Liu, Hailong, Meng, Qinghong, Wang, Zhao, Feng, Chunling, Li, Yuanchun
Format: Article
Language:English
Subjects:
Cancer cells
Cancer therapies
Cell culture
Cervical cancer
Colorectal cancer
Continuous positive airway pressure
Electric wire and cable industry
Enzymes
Fluorescence
Genes
Hospitals
Luciferase
Medical research
Metastasis
MicroRNAs
Oncology
Pathogenesis
Polymerase chain reaction
Proteins
Surgery
Thyroid cancer
Thyroid diseases
Thyroid gland
Time
Tumors
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Summary:This study explored the target of miR-205 and the effect of miR-205 on the proliferation and migration regulating its target in thyroid cancer cells (TC). Twenty-five pairs of TC and adjacent tissues were collected after surgical resection. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of miR-205 in TC tissues and cells (SW579, B-CPAP, TPC-1, WRO). SW579 cells were transfected with miR-205 mimic, and SW579 cells with overexpression of miR-205 were constructed. The effects of miR-205 overexpression on the proliferation and migration of SW579 cells were observed by cell counting kit-8 (CCK-8) and Transwell assays, respectively. Luciferase reporter assay was further used to look for the target of miR-205 and to study the mechanism of miR-205 in the proliferation and migration of TC cells. Compared with normal tissues and cells, the expression of miR-205 was significantly reduced in TC tissues (t=3.47, P=0.031) and cells (t=5.41, P=0.016). Overexpression of miR-205 inhibited the proliferation (t=4.12, P=0.035) and migration (t=4.47, P=0.027) of SW579 cells. Luciferase reporter assays found that CCNB2 was a target gene of miR-205 (t=4.63, P=0.024), qRT-PCR and western blot assays confirmed there was negatively correlation between CCNB2 and miR-205 (t=3.55, P=0.029; t=2.86, P=0.043). CCNB2 overexpression reversed the inhibition of miR-205 on the proliferation (t=3.70, P=0.031) and migration (t=4.12, P=0.022) of SW579 cells. In conclusion, miR-205 inhibits the proliferation and migration of TC cells by targeting CCNB2, which may be a potential target of TC therapy.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2020.11275