Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis

The lncRNA tumor suppressor candidate 8 (TUSC8) plays a critical role in the development of several cancers. However, the biological functions and underlying molecular mechanisms of TUSC8 with respect to breast cancer remain largely unclear. Here, we found that TUSC8 was significantly down-regulated...

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Bibliographic Details
Published in:Aging (Albany, NY.) NY.), 2020-02, Vol.12 (3), p.2974-2991
Main Authors: Zhao, Luqing, Zhou, Yangying, Zhao, Yuelong, Li, Qingling, Zhou, Jianhua, Mao, Yitao
Format: Article
Language:English
Subjects:
Animals
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Mice
Mice, Nude
MicroRNAs - genetics
MicroRNAs - metabolism
Neoplasm Metastasis
Neoplasms, Experimental
Research Paper
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Ubiquitin-Protein Ligases
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Summary:The lncRNA tumor suppressor candidate 8 (TUSC8) plays a critical role in the development of several cancers. However, the biological functions and underlying molecular mechanisms of TUSC8 with respect to breast cancer remain largely unclear. Here, we found that TUSC8 was significantly down-regulated in breast cancer tissues and its high expression predicted better prognosis of breast cancer patients. Functionally, knock-down of TUSC8 drastically promoted the proliferation, migration and invasion of breast cancer cells and facilitated tumorigenicity and metastasis . Mechanistically, the results of luciferase reporter, RIP and RNA pull-down assays proved that TUSC8 functioned as molecular sponge for miR-190b-5p. Furthermore, we showed that TUSC8 served as a competing endogenous RNA (ceRNA) of myosin regulatory light chain interacting protein (MYLIP) through competitively binding with miR-190b-5p and suppressed breast cancer metastasis through regulating the expression of epithelial-mesenchymal transition (EMT) related markers. Clinically, the receiver operating characteristic curve (ROC) analyses revealed that the combination usage of TUSC8 and MYLIP might become novel promising diagnostic biomarkers for breast cancer. Taken together, these results suggested that TUSC8 inhibited breast cancer growth and metastasis via miR-190b-5p/MYLIP axis, providing us new insights into developing potential therapeutic targets for breast cancer patients.
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.102791