Multistep peripherin-2/rds self-assembly drives membrane curvature for outer segment disk architecture and photoreceptor viability

• Published in: Proceedings of the National Academy of Sciences - PNAS 2020-02, Vol.117 (8), p.4400-4410
• Main Authors: Milstein, Michelle L., Cavanaugh, Breyanna L., Roussey, Nicole M., Volland, Stefanie, Williams, David S., Goldberg, Andrew F. X.
• Format: Article
• Language: English
• Subjects: Animals; Architecture; Biological Sciences; Continuity; Curvature; Disks; Evolution; Humans; Mathematical analysis; Membranes; Molecular structure; Morphogenesis; Mutation; Peripherin; Peripherins - chemistry; Peripherins - genetics; Peripherins - metabolism; Phenotypes; Photons; Photoreceptors; Phototransduction; Polymerization; Retina; Retinal Cone Photoreceptor Cells - chemistry; Retinal Cone Photoreceptor Cells - metabolism; Retinal degeneration; Retinal Rod Photoreceptor Cells - chemistry; Retinal Rod Photoreceptor Cells - metabolism; Rod Cell Outer Segment - chemistry; Rod Cell Outer Segment - metabolism; Rod outer segment membranes; Self-assembly; Structural integrity; Structure-function relationships; Transmission electron microscopy; Tubules; Viability; Xenopus laevis
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1912513117

Rod and cone photoreceptor outer segment (OS) structural integrity is essential for normal vision; disruptions contribute to a broad variety of retinal ciliopathies. OSs possess many hundreds of stacked membranous disks, which capture photons and scaffold the phototransduction cascade. Although the molecular basis of OS structure remains unresolved, recent studies suggest that the photoreceptor-specific tetraspanin, peripherin-2/rds (P/rds), may contribute to the highly curved rim domains at disk edges. Here, we demonstrate that tetrameric P/rds self-assembly is required for generating high-curvature membranes in cellulo, implicating the noncovalent tetramer as a minimal unit of function. P/rds activity was promoted by disulfide-mediated tetramer polymerization, which transformed localized regions of curvature into high-curvature tubules of extended lengths. Transmission electron microscopy visualization of P/rds purified from OS membranes revealed disulfide-linked tetramer chains up to 100 nm long, suggesting that chains maintain membrane curvature continuity over extended distances. We tested this idea in Xenopus laevis photoreceptors, and found that transgenic expression of nonchainforming P/rds generated abundant high-curvature OS membranes, whichwere improperly but specifically organized as ectopic incisures and disk rims. These striking phenotypes demonstrate the importance of P/rds tetramer chain formation for the continuity of rim formation during disk morphogenesis. Overall, this study advances understanding of the normal structure and function of P/rds for OS architecture and biogenesis, and clarifies how pathogenic loss-of-function mutations in P/rds cause photoreceptor structural defects to trigger progressive retinal degenerations. It also introduces the possibility that other tetraspanins may generate or sense membrane curvature in support of diverse biological functions.