Noncoding SNPs influence a distinct phase of Polycomb silencing to destabilize long-term epigenetic memory at Arabidopsis FLC

In , the cold-induced epigenetic regulation of ( ) involves distinct phases of Polycomb repressive complex 2 (PRC2) silencing. During cold, a PHD-PRC2 complex metastably and digitally nucleates H3K27me3 within On return to warm, PHD-PRC2 spreads across the locus delivering H3K27me3 to maintain long-...

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Bibliographic Details
Published in:Genes & development 2020-03, Vol.34 (5-6), p.446-461
Main Authors: Qüesta, Julia I, Antoniou-Kourounioti, Rea L, Rosa, Stefanie, Li, Peijin, Duncan, Susan, Whittaker, Charles, Howard, Martin, Dean, Caroline
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis Proteins - genetics
DNA Replication
Epigenesis, Genetic - genetics
Flowers - metabolism
Gene Silencing
Genomic Instability - genetics
Histones - metabolism
MADS Domain Proteins - genetics
Polycomb-Group Proteins - genetics
Polymorphism, Single Nucleotide - genetics
Research Paper
Temperature
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Summary:In , the cold-induced epigenetic regulation of ( ) involves distinct phases of Polycomb repressive complex 2 (PRC2) silencing. During cold, a PHD-PRC2 complex metastably and digitally nucleates H3K27me3 within On return to warm, PHD-PRC2 spreads across the locus delivering H3K27me3 to maintain long-term silencing. Here, we studied natural variation in this process in accessions, exploring Lov-1, which shows reactivation on return to warm, a feature characteristic of in perennial This analysis identifies an additional phase in this Polycomb silencing mechanism downstream from H3K27me3 spreading. In this long-term silencing (perpetuated) phase, the PHD proteins are lost from the nucleation region and silencing is likely maintained by the read-write feedbacks associated with H3K27me3. A combination of noncoding SNPs in the nucleation region mediates instability in this long-term silencing phase with the result that Lov-1 frequently digitally reactivates in individual cells, with a probability that diminishes with increasing cold duration. We propose that this decrease in reactivation probability is due to reduced DNA replication after flowering. Overall, this work defines an additional phase in the Polycomb mechanism instrumental in natural variation of silencing, and provides avenues to dissect broader evolutionary changes at .
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.333245.119