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Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

Immune system alteration has been implicated in the pathogenesis of chronic pain conditions, epilepsy and generalized anxiety disorder. Targeting cytokines has recently been proposed for the management of such conditions. Pregabalin (PGB) is an antiepileptic agent used for the management of these co...

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Published in:Scientific reports 2020-03, Vol.10 (1), p.4007, Article 4007
Main Authors: Abu-rish, Eman Y., Mansour, Ahmad T., Mansour, Hebah T., Dahabiyeh, Lina A., Aleidi, Shereen M., Bustanji, Yasser
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description Immune system alteration has been implicated in the pathogenesis of chronic pain conditions, epilepsy and generalized anxiety disorder. Targeting cytokines has recently been proposed for the management of such conditions. Pregabalin (PGB) is an antiepileptic agent used for the management of these conditions. However, little is known about its immunomodulatory effects on cytokine secretion in vivo and in vitro . Hence, a mitogen (Lipopolysaccharide [LPS] or Concanavalin A [ConA])-induced murine model of inflammation was used to investigate the effect of PGB on in vivo and in vitro IL-1β, IL-6, TNF-α and IL-2 cytokine secretion using ELISA. In addition, PGB effect on spleen histology, as a lymphoid organ, was examined. Our results revealed that PGB significantly inhibited the secretion of ConA-induced IL-6 secretion, basal and ConA-induced TNF-α and IL-2 secretion in splenocytes in vitro . In vivo , PGB inhibited basal and LPS/ConA-induced IL-6 and TNF-α secretion in addition to LPS-induced IL-1β and ConA-induced IL-2 secretion. Moreover, PGB attenuated mitogen-induced inflammatory changes in the spleen. These findings provide an evidence of the anti-inflammatory properties of PGB on cytokine secretion and lymphoid organ inflammation. This might give insights into the role of PGB in the management of the inflammatory state in PGB-indicated conditions.
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subjects 13
13/106
13/21
14/63
631/250/256/2516
631/250/371
64
64/60
692/617/375/178
692/699/375/1692
Animal models
Animals
Anti-inflammatory agents
Antiepileptic agents
Anxiety
Chronic pain
Concanavalin A
Cytokines
Cytokines - immunology
Disease Models, Animal
Enzyme-linked immunosorbent assay
Epilepsy
Female
Histology
Humanities and Social Sciences
IL-1β
Immune system
Immunomodulation
Inflammation
Inflammation - chemically induced
Inflammation - immunology
Inflammation - pathology
Interleukin 2
Interleukin 6
Lipopolysaccharides
Lipopolysaccharides - toxicity
Mice
Mice, Inbred BALB C
multidisciplinary
Pregabalin - pharmacology
Science
Science (multidisciplinary)
Spleen
Spleen - immunology
Spleen - pathology
Splenocytes
Tumor necrosis factor-α
title Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation
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